Comparison of gene expression levels between ONHs in D2.hGFAPpr-EGFP mice (A, B and C) and ONHs after crush (A’, B’ and C’).

<p>Gene expression levels in the D2 mice were determined by qRT-PCR. Each independent sample contained 3–4 ONHs with the same retina and optic nerve grades. There were total 2 no glaucoma (Nog), 3 moderate glaucoma (Mod) and 3 severe glaucoma (Sev) samples. The expression levels of each gene were normalized to the mean of the no glaucoma samples. Gene expression levels after crush were from the microarray data and the expression levels of the crushed eyes were normalized to the contralateral controls. Results were presented as mean with 90% lower and upper confidence bounds. Many genes showed similar responses after these two injuries. The genes that had different responses were highlighted with black frames.