Markers to identify cell lineages within <i>in vitro</i> cultures.

<p>The identification of specific cell lineages within <i>in vitro</i> cultures of porcine crypts is shown. The existence of stem/progenitor and differentiated lineages were confirmed in enteroids utilizing the established genetic biomarkers for cell lineage identification: anti-SOX9 (stem/progenitor), anti-PCNA (proliferation), anti-CgA (enteroendocrine), anti-MUC2 (goblet) and anti-sucrase isomaltase (absorptive enterocyte) antibodies. All specific markers (red). Nuclei, blue. Scale Bar 50 µm, inset scale bar 10 µm.