hTP Functional Analysis in PLP.

<p><b>A.</b> Meg-01 Cells were nucleofected with hTP. Surface adherent megakaryocyte stained for p-Selectin (green) surrounded by produced platelet-like particles over-expressing nucleofected RFP-hTP-alpha. <b>B.</b> Stimulated display of CD62p (P-selectin) upon U-46619 treatment (100 nM) was reduced for the dysfunctional V⁸⁰E variant, as well as completely unresponsive for the known bleeding variant R⁶⁰L and D304N. Both baseline and stimulated levels of P-selectin were higher than wild type for the A¹⁶⁰T variant, suggesting a considerable degree of constitutive activity. At least three independent repetitions were performed for each construct. Shown are mean ± SEM.