Figure 2

<p>No MARCO expression is measured in DCs generated from the bone marrow of MARCO^-/- mice. A) RT-PCR analysis of MARCO expression in DCs generated from the bone marrow of wild-type or knockout mice. DCs were unpulsed, pulsed with B16 tumor lysate, or pulsed with 1 µg/ml LPS for 24 hours. Amounts of mRNA were adjusted to give comparable GAPDH signals. B) DCs from wild-type or knockout mice were pulsed for 24 hours with PKH26-red labeled tumor lysate (red). DCs were stained for MARCO expression (green) and DAPI (blue).