AT₂ receptor protein expression in adrenal, spleen, kidney and brainstem of wild-type and AT₂ knockout mice.

<p>Angiotensin II AT₂ receptor protein expression was studied by western blotting. Protein extracts were separated by SDS-PAGE electrophoresis and exposed to three different anti-AT₂ receptor antibodies as indicated by catalog number at the top of the picture. The scale on the left indicates the size in kDa according to the positions of the protein ladder. The reported size of the AT₂ receptor is about 50-55 kDa. Note that each antibody tested generated different immunoreactivity patterns, that these patterns do not correlate with the presence or absence of the target protein, and that in all cases there is no difference between band intensity obtained from tissues from AT₂ knockout and wild-type mice. The figure represents a typical experiment repeated two times in individual samples.