The effect of CDF on adult female egg-laying.

<p>Chemically defined food extends the egg-laying ability of adult female flies. (A) Average egg-lay in 12 hours of adult female flies on chemically defined food as a function of caloric density. Females fed on CDF show an increase in both maximal egg-lay and reproductive lifespan (n = 4). B) Maximal 12-hour egg-lay on chemically defined food. CDF^100K–400K enhances the maximal egg-laying ability compared to RF (Mann Whitney test; n = 4, p = 0.2454 for CDF^500K and 0.0286 for others). (C) Number of days a female is capable of producing more than one egg per day. CDF^100K–400K extends the reproductive lifespan compared to RF (Mann Whitney test; n = 4, p = 0.1441 for CDF^500K and ≤0.0294 for others). (D) Total lifetime egg-lay per female on chemically defined food. Females lay more eggs on CDF^100K–400K than on RF (Mann Whitney test; n = 4, p = 0.0571 for CDF^500K and 0.0286 for others). See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067308#pone.0067308.s005" target="_blank">Table S3</a> for additional details.