Increased osteogenic differentiation and extracellular matrix calcification in MSCs from <i>APN</i> KO mice.

<p>(A) RT-PCR shows that mRNA expression of the indicated genes (left). The respective ratio of band density (KO/WT, %) normalized with <i>β-actin</i> represents relative mRNA levels (right). A representative result of three independent experiments is shown. (B) Increased ALP activity in MSC lysate from <i>APN</i> KO mice. (C) Alizarin red S staining shows enhanced calcification ability of MSCs from <i>APN</i> KO mice (n = 3 for each). (D) Ca²⁺ content in cell lysate from <i>APN</i> KO mice is higher than that from WT mice (n = 3 for each). **indicates <i>p</i><0.01.