NE enhances TNF-α production from BMM.

<p>Bone marrow cells were cultured with M-CSF in the presence of NE (1 x 10^-8 M and 1 x 10^-6 M). At day 7, LPS (50 ng/mL) and the protein transport inhibitor GolgiPlug (final concentration, 1 µg/mL) were added to the culture for further 4 hour culture and then cells were collected and sequentially stained with membrane antibodies for CD11b and F4/80, and intracellular antibody of TNF-α. Representative flow cytometric data was shown in (A) and corresponding graphic data in (B). Graph bars represent TNF-α⁺ BMM as the means ± SD of 2 independent experiments in triplicate each time. ** p<0.01, compared to untreated control (0 M).