Knockdown of Gdf15 or Scgb3a1 impairs myoblast fusion.

<p>C2C12 myoblasts were transduced overnight with lentiviruses expressing shRNAs for Gdf15 (A-C) or Scgb3a1 (D-E) as described in Fig. 2 legend. (<b>A</b>) MHC (green) and DAPI (red) staining of Gdf15 knockdown cells at the end of 3-day differentiation. (<b>B</b>) Quantification of myotube formation shown in A. (<b>C</b>) RT-PCR results for Gdf15 mRNA. (<b>D</b>) MHC (green) and DAPI (red) staining of Scgb3a1 knockdown cells at the end of 3-day differentiation. (<b>E</b>) Quantification of myotube formation shown in D. Data shown are mean ± SD (n = 3). One sample (C) or paired (B & E) t tests were performed to compare data to control. *P<0.05. **P<0.01. Scale bars: 100 µm.