NMU mRNA-expressing cell types in the PT.

<p>Microphotograph of NMU mRNA expression detected by double staining by HNPP fluorescent ISH for NMU mRNA and by fluorescent IHC for TSH in fixed, frozen 5-µm sections. (A) Microphotograph of NMU mRNA expression detected by HNPP fluorescent ISH (red). NMU mRNA-expressing cells were widely distributed in the PT. (B) Microphotograph of TSH detected by fluorescent IHC staining. Many TSH-immunostained cells were distributed throughout the PT (green). (C) Microphotograph of merged pictures of double staining for NMU mRNA expression, which was detected by HNPP fluorescent ISH and TSH detected by fluorescent IHC. Yellow color represents the merged signal for NMU mRNA and TSH production. Although almost all NMU-expressing cells showed TSH immunoreactivity (arrows, yellow), cells exhibiting only TSH immunostaining were also found (arrowheads, green). (D) Magnified image of the boxed area in C. NMU mRNA-expressing and TSH producing cells (arrows, yellow) and cells producing only TSH (arrowheads, green) exist. Scale bars: 50 µm. PT, pars tuberalis; ME, median eminence.