Placental STAT6 and IL-4 levels are decreased significantly in P-PIC mice.

<p>A. Schematic of human <i>STAT6</i> 3′-UTR sequence targeted by miR-210. The predicted seed regions by both TargetScan and miRanda algorithms are indicated. B. Placental cell lysates from both P and P-PIC mice at gestational day 18 were subjected to immunoblot analyses using anti-STAT6 antibodies. Immunoblot analysis with an anti-β-actin antibody served as a loading control. The first lane in the immunoblot indicates the molecular weight marker. C. mRNA levels of IL-4 were determined by qRT–PCR after isolation of total RNA from placentas of both P and P-PIC mice at gestational day 18. qRT–PCR of GAPDH was used for normalization. Results are expressed as mean+SEM for 3 independent experiments. *<i>P</i><0.05 vs. P.