<p>(a) Sequence of the CL14-U/CP25 substrate and highlight of the Top1 cleavage site containing a scissile ribonucleoside monophosphate. (b) Time course (0.25-15 minutes) of the cleavage reaction of purified hTop1 (lanes 1-8), or hTop1(pf-Linker) (lanes 9-16) with the CL14-U/CP25 substrate (shown in a). In lane 15 the protein has not been added. Cl1 represents the DNA substrate cleaved by the enzymes at the preferred cleavage site. (c) Percentage of cleaved substrate plotted against time for the reaction with hTop1 (black) and with hTop1(pf-Linker) (grey). Data shown are means ± SD from 3 independent experiments.</p
<p>The concentration of indicated protease fragments and the substrates xLC3B-MBP (left) or xGATE16-...
This thesis reports the results of experiments designed to help elucidate the catalytic mechanisms o...
<p>Representative SDS-PAGE of hproNGF WT (panel A), hproNGF P61S (panel B), hproNGF P61SR100E (panel...
<p>(a) Time course (0.5-60 minutes) of the cleavage reaction of the purified hTop1 (lanes 1-8), or h...
<p>(a) Gel electrophoresis of the products generated by incubation of hTop1 (lanes 1-8) and hTop1(pf...
<p>(a) Gel analysis of the religation kinetics observed when incubating the hTop1- or hTop1(pf-Linke...
AbstractWe compared the cleavage by a hammerhead ribozyme of a wild-type precursor tRNA (pre-tRNA3le...
<p>Panels A and B show an analysis of the P1´and P2´specificities. The sequences of the different su...
The extracellular nuclease from Serratia marcescens is a non-specific endonuclease that hydrolyzes d...
<p>Substrate (20 nM) was incubated alone (lanes 1), with 1 nM of V38-TAT (lane 2), 5 nM of M1-TAT (l...
<p><b>Copyright information:</b></p><p>Taken from "Investigation of Mg- and temperature-dependent fo...
<p>(<b>A</b>) Autoradiogram of the denaturing PAGE gel used for the analysis of the cleavage reactio...
<p><b>(a)</b> Diagrammatic representation of the DNA substrates used: pUC19 (left) and pTK-neo (righ...
<p>Panels A to D shows the cleavage of a number of substrates by thrombin, where individual amino ac...
ABSTRACT: A kinetic mechanism is presented for the cleavage of Bacillus subtilis precursor tRNAAsp c...
<p>The concentration of indicated protease fragments and the substrates xLC3B-MBP (left) or xGATE16-...
This thesis reports the results of experiments designed to help elucidate the catalytic mechanisms o...
<p>Representative SDS-PAGE of hproNGF WT (panel A), hproNGF P61S (panel B), hproNGF P61SR100E (panel...
<p>(a) Time course (0.5-60 minutes) of the cleavage reaction of the purified hTop1 (lanes 1-8), or h...
<p>(a) Gel electrophoresis of the products generated by incubation of hTop1 (lanes 1-8) and hTop1(pf...
<p>(a) Gel analysis of the religation kinetics observed when incubating the hTop1- or hTop1(pf-Linke...
AbstractWe compared the cleavage by a hammerhead ribozyme of a wild-type precursor tRNA (pre-tRNA3le...
<p>Panels A and B show an analysis of the P1´and P2´specificities. The sequences of the different su...
The extracellular nuclease from Serratia marcescens is a non-specific endonuclease that hydrolyzes d...
<p>Substrate (20 nM) was incubated alone (lanes 1), with 1 nM of V38-TAT (lane 2), 5 nM of M1-TAT (l...
<p><b>Copyright information:</b></p><p>Taken from "Investigation of Mg- and temperature-dependent fo...
<p>(<b>A</b>) Autoradiogram of the denaturing PAGE gel used for the analysis of the cleavage reactio...
<p><b>(a)</b> Diagrammatic representation of the DNA substrates used: pUC19 (left) and pTK-neo (righ...
<p>Panels A to D shows the cleavage of a number of substrates by thrombin, where individual amino ac...
ABSTRACT: A kinetic mechanism is presented for the cleavage of Bacillus subtilis precursor tRNAAsp c...
<p>The concentration of indicated protease fragments and the substrates xLC3B-MBP (left) or xGATE16-...
This thesis reports the results of experiments designed to help elucidate the catalytic mechanisms o...
<p>Representative SDS-PAGE of hproNGF WT (panel A), hproNGF P61S (panel B), hproNGF P61SR100E (panel...