Effect of UBB⁺¹ on IL-1β- and TNF-α-mediated chemokine expression.

<p>CRT-MG cells stably expressing pEGFP or pEGFP-UBB⁺¹ were treated with varying doses of TNF-α or IL-1β for 24 h, and the supernatant was assayed for CCL-2 (A) or CXCL-8 (B) protein by ELISA. Control samples are the supernatants from the cells without any treatment. Representative of two independent experiments. Cells stably expressing pEGFP or pEGFP-UBB⁺¹ were treated with IL-1β (4 µg/L) or TNF-α (10 µg/L) for 30 min, and then the nuclear extracts from cells were assayed for the binding activity of p65/c-Rel (NF-κB) (C) or c-Jun (AP-1) (D). Values statistically significantly different from the value for the control samples without any treatment are indicated by asterisks (*, p<0.05; **, p<0.01; and ***, p<0.001). Representative of two independent experiments.