Verification of PSMB1 expression in PA under prolonged hypoxia.

<p><b>A:</b> Zoomed-in view of protein spot 28 in the 2DE gel in Fig. 1. <b>B:</b> Relative protein expression of PSMB1 in the 2DE gel. The normoxia group was considered as 100% and the relative fold-change in protein level is shown by spot density. <b>C:</b> Relative mRNA levels for PSMB1 from the PA of rats exposed to 10% O₂ for 21 days compared with normoxia control. Bar values are mean ± SEM (n = 5 in each group). *<i>P</i><0.05 versus respective normoxia control. <b>D:</b> Protein levels of PSMB1 measured by Western blot in PA isolated from rats exposed to hypoxia (10% O₂, 21 days) or normoxia. <b>E:</b> Band intensity of PSMB1 normalized to α-actin. Mean values ± SE were calculated from three independent samples. *<i>P</i><0.01 versus normoxia.