Effects of sCJD and vCJD brain dilution on PrP^Sc detection by SOFIA.

<p>Ten percent homogenates from all the normal human (N), vCJD (V), and sCJD brain tissues were prepared as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066352#s4" target="_blank">Materials and Methods</a>. Serial 10-fold dilutions were prepared from each brain tissue sample and analyzed in triplicate by SOFIA as described. For each sample, data was calculated as the mean fluorescent signal±standard deviation and expressed as the sample to background ratio (signal intensity).