Rapamycin suppresses mTOR phosphorylation at Ser-2448 in a dose dependent manner.

<p>Primary cortical neurons were treated under normoxic conditions with various concentrations of rapamycin (0 nM – 20 nM). (A,B) Rapamycin caused a dose-dependent decrease in phosphorylated mTOR at Ser-2448, and subsequently caused a dose-dependent decrease in phosphorylated p70S6K. (C,D) Rapamycin at 20 nM caused a small decrease in phosphorylated mTOR at Ser-2481. The lower concentrations of rapamycin (2 nM – 10 nM) increased the phosphorylation of Akt, whereas 20 nM of rapamycin caused a decrease in phosphorylated Akt. (A,B) Representative immunoblots. (B,D) Quantitative analysis is shown as percent control for all phospho-proteins normalized to total protein. Data is represented as the mean ± s.e.m. (n = 4). *p<0.05 vs. 0 nM, #p<0.05 10 nM vs. 20 nM.