Increase of Hsp70 protein expression in Cry1Ab-treated IPEC-J2 cells.

<p>IPEC-J2 cells were treated with Cry1Ab for 24 hours and the Hsp70 protein was determined by ELISA and Western blot (insert). For ELISA Hsp70 is normalized to µg protein extract used in the assay. The normalized data are represented as mean relative to the control. Data represent the mean +/− SD; n = 5 different experiments; (*) results are significantly different as compared with untreated controls (P<0.05). For Western blotting the membranes were additionally incubated with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mouse monoclonal antibody (Abcam), which serves as control for protein expression.