Pair distance distribution functions for the protein samples during fibrillation.

<p>Data are for (A) aSN wt and (B) A30P. Colours are as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067713#pone-0067713-g003" target="_blank">Fig. 3</a>. Inserts depict the development in D_max, (filled symbols) versus time, plotted together with the development in the ThT signal (open symbols). It is evident that the maximally occurring D_max is present already at early time-points in both wt and mutant protein fibrillation. Likewise it can be seen that there is an increase in the observed D_max before an onset of fibrillation is registered via increas in the ThT intensity. The pair distance distribution functions are normalized to the same area under the curve, for better visualization of small species. The insert shows an increase in the maximum pair distance (filled symbols) prior to observed increases in ThT fluorescence (open symbols).