Liver growth factor modulated the expression of phospho-Akt and Bcl2 in the striatum and mesencephalon of 6-OHDA-lesioned rats.

<p>As shown in A, the decrease in phospho-Akt/Akt ratio observed in vehicle-treated rats (A, white bar) was prevented 24 hours after the administration of a single injection of LGF (A, lined bar). In the lesioned mesencephalon, the phospho-Akt/Akt ratio was significantly increased 72 hours after the beginning of LGF treatment (B, lined bars), as compared with control (B, dotted bar), and the lesioned mesencephalon of vehicle-treated rats (B, white bar). Panels C and D show Bcl2/Bax ratios in the lesioned striatum (C) and mesencephalon (D) of 6-OHDA-lesioned rats. Note how a single injection of LGF promoted a transitory, but significant, increase in Bcl2/Bax ratio in both structures. Lane 1: control; lane 2: vehicle-treated rats; lane 3∶24-hour LGF-treated rats; lane 3″: lesioned mesencephalon of 48-hour LGF-treated rats; lane 4∶72-hour LGF-treated rats. Results are expressed as percentage of control (naïve striatum and mesencephalon of IP-vehicle treated rats at 13 weeks post-lesion), and represent the mean ± SEM of n individual rats. One way ANOVA were performed in A (p = 0.0064; F_{3, 26} = 5.135, n = 6–11), B (p = 0.014; F_{3, 22} = 4.426, n = 6–7), C (p = 0.0009; F_{3, 22} = 8.003, n = 6–7) and D (p = 0.0047; F_{3, 23} = 5.651, n = 6–7) followed by Newman-Keuls multiple comparison test (*p≤0.05 and **p≤0.01 vs control. +p≤0.05 and ++p≤0.01 vs vehicle. °°p≤0.01 vs 24 hours post IP-LGF. ··p≤0.01 vs 72 hours post-LGF).