Absence of M029 enhances MYXV sensitivity to Type I IFN.

<p>Human A549 cell lines with constitutive knockdown of PKR (shPKRA549) or a control (shControl A549) were constructed as described for HeLa. The cells were infected with vMyx-GFP (top panel) or vMyx-M029ID with or without treatment of human IFNβ (1000 U/ml). A) Fluorescence images were taken after 48 h p.i. with an MOI of 0.1. B) Virus was tittered after 1 and 72 h of p. i. using RK13-E3 cells. C) A549 cells were infected with VSV with an MOI of 0.1 with or without treatment of human IFNβ (1000 U/ml). Virus was tittered after 24 h p.i. using A549 cells to determine the titer. Data are mean ± SD of triplicate samples from one experiment and are representative of two independent experiments. **<i>P</i><0.01 compared with vMyx-M029ID infection.