Sequence trace comparison identifies the target base and minimal recognition sequence of MamA.

<p>Plasmids containing putative MamA-recognition motifs were propagated in the indicated bacterial strains, isolated and sequenced. Sequence traces shown are representative of at least 2–3 biological replicates. (A) The 10 base pair sequence shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat-1003419-g001" target="_blank">Figure 1E</a> supports methylation of one adenine on each strand in wildtype H37Rv, as evidenced by increased thymine peak areas relative to the identical sequence context in <i>E. coli</i> and methylation-deficient strains of <i>M. tuberculosis</i>. See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat.1003419.s002" target="_blank">Figure S2</a> for quantification of peak areas. (B) Schematic depiction of the positions of N⁶-methyladenine residues. (C) A six base pair core sequence is sufficient to direct MamA-mediated methylation (bold in panel B). See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat.1003419.s008" target="_blank">Table S1</a> for a complete list of tested sequences. (D) Positions of MamA recognition sequences are shown schematically on the 4.4 Mb <i>M. tuberculosis</i> genome.