Effects of butyrate and FBA on liver steatosis.

<p>Paraffin-embedded sections of the liver (n=4 each group) were stained with hematoxylin-eosin (A) or oil red O (B). Micrographs in both panels are representative pictures with original magnification 40X. Foci of inflammatory cells are shown (arrows). (C) Triglycerides were measured in liver and normalized on 500 mg of frozen tissue (n=6 each group). The mRNA levels of pro-inflammatory cytokines TNF-α (D), MCP-1 (E), F4/80 (F), and TGF-β (G) were analyzed by real-time PCR in liver extracts (n=6, each group). *P<0.05, ***P<0.001 <i>vs</i> STD; ^# P<0.05, ^{# #} P<0.01, ^{# # #} P<0.001 <i>vs</i> HFD.