ISL inhibited VEGF-induced tube formation, invasion and migration.

<p>(A) HUVECs were seeded at a density of 1×10⁴ cells/well per 24-well plate. Plates were previously coated with Matrigel and stimulated with VEGF (20 ng/ml) in the presence or absence of ISL (a: 0; b: 5 µM, c: 10 µM; d: 20 µM) for 12 h. The results showed that ISL significantly abrogated the formation of capillary network; (B) HUVECs at a density of 5×10⁵ cells/ml were plated onto the upper membrane of 6-well transwell coated with Matrigel. After 24 h, cells that invasive to the opposite side of the membrane were counted (a: 0; b: 5 µM, c: 10 µM; d: 20 µM). The results revealed that ISL decreased invasive ability of HUVECs in a dose-dependent manner; (C) HUVECs at a density of 5×10⁵ cells/ml were seeded in a 6-well plate for wound healing assay. The results showed that ISL (20 µM) significantly inhibited endothelial cells migration under the stimulation of VEGF (All values represented as mean ± SD, n = 3, *<i>P</i><0.05 <i>versus</i> untreated control).