Purification table for recombinant SH variant 2 (six-subunit enzyme with 5′-Strep tagged HoxI), purified from recombinant <i>E. coli</i> strain SH2F cells.

  • Johannes Schiffels (431126)
  • Olaf Pinkenburg (431127)
  • Maximilian Schelden (431128)
  • El-Hussiny A. A. Aboulnaga (431129)
  • Marcus E. M. Baumann (431130)
  • Thorsten Selmer (431131)
Publication date
July 2013

Abstract

<p>About 7 g of wet packed cells were used for purification.</p>a<p>H<sub>2</sub>:NAD<sup>+</sup>physiological activity, measured under anaerobic conditions. 1 Unit is defined as the H<sub>2</sub>-mediated reduction of 1 µmol NAD<sup>+</sup>per minute.</p

Extracted data

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