<p>About 7 g of wet packed cells were used for purification.</p>a<p>H<sub>2</sub>:NAD<sup>+</sup>physiological activity, measured under anaerobic conditions. 1 Unit is defined as the H<sub>2</sub>-mediated reduction of 1 µmol NAD<sup>+</sup>per minute.</p
<p>(A) SDS-PAGE analysis of purified recombinant NfBGL1. Lanes: 1, the molecular mass standards; 2, ...
a<p>From 0.84 g of <i>B. subtilis</i> cell paste obtained from 300 ml of bacterial culture.</p>b<p>P...
<p>Typical 12% SDS-PAGE analysis of recombinant His-tagged bioreactor-produced proteins including Lp...
<p>About 20 g of wet packed cells were used for purification.</p>a<p>H<sub>2</sub>:NAD<sup>+</sup>ph...
<p>Cells from strain SH2F (six-subunit SHvar2; 5′-StrepII tagged HoxI; HoxI*), cultivated under ‘opt...
a<p>The starting material was 250 ml of crude <i>E. coli</i> supernatant.</p>b<p>One milliunit (mU) ...
<p>The reaction mixture contained 100 mM Tris-HCl (pH 8.0), 30 µM horse heart cytochrome <i>c</i>, 1...
a<p>The starting material was 250 ml of crude <i>E. coli</i> supernatant.</p>b<p>One milliunit (mU) ...
<p>M, protein marker; lanes 1 and 2, negative controls of untransformed vector and recombinant <i>E....
<p>(A) Reduced CO-difference spectrum of recombinant CYP2D49 (solubilized membrane fraction). The sp...
In pyridine nucleotide-dependent, reductive whole cell biotransformation with resting cells of Esche...
Additional file 1: Figure S1. Purification of recombinant C. jejuni HtrA expressed in E. coli BL21. ...
(A) The size-exclusion chromatogram of SOR using Superdex 200 10/300 GL column (GE Healthcare). (B) ...
AbstractBy combining extracts from strains lacking genes encoding either the maturation enzymes or t...
The current study has been aimed at isolating and purifying appropriate amounts of the human recombi...
<p>(A) SDS-PAGE analysis of purified recombinant NfBGL1. Lanes: 1, the molecular mass standards; 2, ...
a<p>From 0.84 g of <i>B. subtilis</i> cell paste obtained from 300 ml of bacterial culture.</p>b<p>P...
<p>Typical 12% SDS-PAGE analysis of recombinant His-tagged bioreactor-produced proteins including Lp...
<p>About 20 g of wet packed cells were used for purification.</p>a<p>H<sub>2</sub>:NAD<sup>+</sup>ph...
<p>Cells from strain SH2F (six-subunit SHvar2; 5′-StrepII tagged HoxI; HoxI*), cultivated under ‘opt...
a<p>The starting material was 250 ml of crude <i>E. coli</i> supernatant.</p>b<p>One milliunit (mU) ...
<p>The reaction mixture contained 100 mM Tris-HCl (pH 8.0), 30 µM horse heart cytochrome <i>c</i>, 1...
a<p>The starting material was 250 ml of crude <i>E. coli</i> supernatant.</p>b<p>One milliunit (mU) ...
<p>M, protein marker; lanes 1 and 2, negative controls of untransformed vector and recombinant <i>E....
<p>(A) Reduced CO-difference spectrum of recombinant CYP2D49 (solubilized membrane fraction). The sp...
In pyridine nucleotide-dependent, reductive whole cell biotransformation with resting cells of Esche...
Additional file 1: Figure S1. Purification of recombinant C. jejuni HtrA expressed in E. coli BL21. ...
(A) The size-exclusion chromatogram of SOR using Superdex 200 10/300 GL column (GE Healthcare). (B) ...
AbstractBy combining extracts from strains lacking genes encoding either the maturation enzymes or t...
The current study has been aimed at isolating and purifying appropriate amounts of the human recombi...
<p>(A) SDS-PAGE analysis of purified recombinant NfBGL1. Lanes: 1, the molecular mass standards; 2, ...
a<p>From 0.84 g of <i>B. subtilis</i> cell paste obtained from 300 ml of bacterial culture.</p>b<p>P...
<p>Typical 12% SDS-PAGE analysis of recombinant His-tagged bioreactor-produced proteins including Lp...