Effect of the induction of P<i>lasR</i>-bound proteins on P<i>lasR</i> promoter activity.

<p>(A) Histogram reporting P<i>lasR</i> maximal promoter activity (grey bars) and the corresponding cell density (white bars) measured in <i>P. aeruginosa</i> PAO1 P<i>lasR::lux</i> strains carrying the plasmids indicated below the graph, grown in LB supplemented with 0.1% (w/v) l-arabinose. (B) Graph reporting P<i>lasR</i> promoter activity (filled symbols) and cell density (open symbols) measured during the growth curve in <i>P. aeruginosa</i> PAO1 P<i>lasR::lux</i> carrying pHERD30T (triangles) or pR3699 (circles), grown in LB supplemented with 0.1% (w/v) l-arabinose. (C) Histogram reporting P<i>lasR</i> maximal promoter activity measured in <i>P. aeruginosa</i> PAO1 P<i>lasR::lux</i> strains carrying pHERD30T (grey bars) or pR3699 (white bars) grown in LB supplemented with different l-arabinose concentrations (%, w/v), indicated below the graph. In (A), (B) and (C) the average of three independent experiments is reported with standard deviations; in (A) and (C) statistical significance with respect to <i>P. aeruginosa</i> PAO1 P<i>lasR::lux</i> (pHERD30T) is indicated with one asterisk (<i>p</i> < 0.01).