Temperature increase worsened sodium azide/2-DG induced reduction of cellular volume.

<p>Cells received different treatments for 1 hour, and then cell volume was measured by BD FACScan flow cytometer with an aid of Cell Quest Pro. The data were acquired using FCS with voltage E00. A total of 50,000 cells were analyzed per sample. Flow cytometry analysis showed smaller forward scatter value following the treatment with sodium azide/2-DG or increased temperature alone. Combination treatment of sodium azide/2-DG and increased temperature produced addictive actions in the decrease of forward scatter value. Data were representative of one of three independent experiments. w/: with; w/o: without.