Recruited inflammatory monocytes give rise to αE-DC in the draining PLN during TB.

<p>(A) Primary monocytes were adoptively transferred and identified in infected recipient mice as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069287#pone-0069287-g001" target="_blank">Figure 1</a> (upper left panels). The myeloid cell subsets were identified based on CD11b and CD11c expression profile (labeled [A]-[D], upper right panels). (B) The myeloid cell subsets were analyzed further for CD103, Ly6C and MHC class II cell surface expression. CD103-expressing Ly6C^-MHC class II⁺ αE-DC were found within the CD11b^-CD11c⁺ population (upper panels). The graph shows MHC class II cell surface expression levels (mean ± SEM) on gated transferred and endogenous cells. *, p<0.05; **, p<0.01; ***, p<0.001 by one-way ANOVA with Bonferroni posttest. Solid lines denote comparisons between transferred cells and dotted lines denote comparisons between endogenous cells. Monocyte differentiation in <i>M. tuberculosis</i>-infected recipient mice was analyzed in two separate experiments with five recipient mice in each experiment.