Effect of increasing PCR inhibition on the accuracy of the HE and LE amplification systems.

<p>PCR quantifications were performed using HE and LE systems in the presence of an equal starting number of template molecules and increasing blocked primer concentrations. Panels (A) and (B) show the obtained amplification plots for each system. Panels (C) and (D) show the relative errors obtained using <i>Cy0</i> and <i>Cy0</i> corrected following Eq. 10. The relative error was reported as <i>Log(DNA_exp)</i>–<i>Log(DNA_obs)</i> where <i>Log(DNA_exp)</i> is the number of expected molecules and <i>Log(DNA_obs)</i> is the number of calculated molecules using the <i>Cy0 (Black dots)</i> or <i>Cy0_corrected (White dots).</i> Each symbol represents a mean 6 runs. SOD detected almost all the runs in which the blocked primer was added as outliers.