Architecture of human PGC-1α and constructs used in this study.

<p>PGC-1α contains three LxxLL motifs, two of which (L2 and L3) are responsible for specific interaction with NRs. The protein constructs used here are PGC-1α RID1 (122–235), PGC-1α RID2 (122–285) and PGC-1α NTD (1–285) that contain motifs L2 and L3. On the right, the sequence of the His₆-tagged PGC-1α RID2 is given showing residues of the L2 and L3 motifs (bold, blue). Residues identified by NMR as being outside the interacting region are shown in green; residues unambiguously identified as being in the interacting region are shown in red; additional candidate Ser/Thr residues, affected by binding are underlined.