Replication capacity in MDM.

<p>The bars represents the percentages of HIV-1 isolate replication in MDM cultures infected with 8,000 pg per 10⁵ cells after 14 days post infection. The percentage of viral replication of each isolate is calculated considering the replication of the control virus HIV_BaL as 100% (median value of 162,143 pg/ml). Mean values of at least 3 experiments for each isolate are shown. Error bars indicating inter-experimental standard deviations are shown. Linear regression model and Pearson correlation coefficient were used to determine the strength of correlation between the replication capacity (p24 production) and genotypic tropism (values of Geno2Pheno FPR). Mann-Whitney-Wilcoxon (MWW) test was used to compare the replication capacity between isolates with FPR <20% and >20%. R² = Coefficient of determination; rho = Pearson correlation coefficient; p, two-tailed p value. The False Positive Rate (FPR) values are based on the V3 sequences and calculated with Geno2Pheno algorithm. The phenotypic tropism was evaluated on astroglioma U87MG-CD4⁺-CCR5⁺−/CXCR4⁺-expressing cells (DR: R5⁺X4; D: R5/X4; DX: R5/X4⁺).