Comparison of HIV-DNA and viral replication in PBMC and CD4+ T-cells.

<p>The bars represents the percentage of HIV-1 DNA copies and p24 values (normalized per 10⁶ cells) in human primary cell types. Panel A and B: PBMC and CD4+ T-cells infected with 16,000 pg per 10⁶ cells after 7 days post infection, respectively. The percentage of p24 production and DNA copies of each isolate is calculated considering the replication of the control virus HIV_BaL as 100 (in PBMC: median p24 production of 45.506 pg/10⁶ and 555.000 HIV DNA copies/10⁶; in CD4+ T-cells: median p24 production of 357.561 pg/10⁶ and 2.5×10⁶ HIV DNA copies/10⁶ cells). The experiments for PBMC and CD4+ T-cells were done using cells from the same donor. Mean p24 production values of 3 biological replicates for each isolate are shown. Error bars indicating intra-experimental standard deviations are shown. Linear regression model and Pearson correlation coefficient were used to determine the strength of correlation between p24 production and HIV-DNA copies. R² = Coefficient of determination; rho = Pearson correlation coefficient; p, two-tailed p value. The False Positive Rate (FPR) values are based on the V3 sequences and calculated with Geno2Pheno algorithm. The phenotypic tropism was evaluated on astroglioma U87MG-CD4⁺-CCR5⁺−/CXCR4⁺-expressing cells (DR: R5⁺X4; D: R5/X4; DX: R5/X4⁺).