SFR1-A enhances ERα and AR transcriptional activity.

<p>The effects of SFR1 overexpression on ERα (A and B), ERβ (D) and AR (C) transcriptional activity were assessed by luciferase reporter assays. Ishikawa cells were co-transfected with nuclear receptors (ERα, ERβ or AR), their respective reporter genes (3xERE-luc and ARE-luc), and indicated amount of p3xFLAG-SFR1-A, p3xFLAG-SFR1-B (20 ng) or p3xFLAG-SFR1-C (20 ng) (D). The cells were treated with vehicle or 1 nM E2 or DHT. Transcriptional activity was measured via luciferase assays at 12 hours and 24 hours after ligand treatments. All luciferase reporter assays were conducted at 12 h after the ligand treatment unless it is indicated in the experiment. 20 ng of SFR1A, SFR1B and SFR1C was transfected unless the amount of DNA was indicated in the figures. All experiments were performed multiple times with triplicate samples. The error bars indicate standard deviations. * = P<0.05; ** = P<0.01; *** = P<0.005.