Establishment of LLC tumor model with luciferase stable expression.

<p>(<b>A</b>) Bioluminescence images of LLC tumors on day 7, 14 and 21 after Luc-LLC subcutaneous inoculation (n = 6); (<b>B</b>) Measurements of photons per second depicting the tumor volumes of mice using the IndiGo imaging analysis software, ^*<i>P</i><0.05, ^**<i>P</i><0.01 <i>vs</i> day 7; (<b>C</b>) Tumor growth curve from day 5 to 25 after Luc-LLC subcutaneous inoculation (n = 6), ^*<i>P</i><0.05, ^**<i>P</i><0.01 <i>vs</i> day 5; (<b>D</b>) Schematic representation of experiment protocol described in materials and methods, animals were divided into four groups: (a) Control; (b) VEGF-Trap alone; (c) Gemcitabine alone; (d) Combination of VEGF-Trap and Gemcitabine.