Bradykinin-stimulated prostanoid accumulation in the circulation <i>in</i><i>vivo</i> in wild-type, <i>Cox1</i><sup><i>-/-</i></sup>, and <i>Cox2</i><sup><i>-/-</i></sup> mice.
- Nicholas S. Kirkby (229832)
- Anne K. Zaiss (432206)
- Paula Urquhart (432207)
- Jing Jiao (121723)
- Philip J. Austin (432208)
- Malak Al-Yamani (432209)
- Martina H. Lundberg (332728)
- Louise S. MacKenzie (432210)
- Timothy D. Warner (200303)
- Anna Nicolaou (261799)
- Harvey R. Herschman (245626)
- Jane A. Mitchell (128611)
DOIAbstract
<p>Accumulation of the stable prostacyclin breakdown product, 6-keto-PGF<sub>1α</sub> in plasma after bradykinin administration (100nmol/kg i.v.) is dependent on COX-1 but not COX-2 when measured by LC-MS/MS (a). Representative LC-MS/MS chromatograms show the presence or absence of 6-keto PGF<sub>1α</sub> in all sample types (retention time 2.81 min; transition ion <i>m</i>/<i>z</i> 369>163). Similar data were obtained for plasma levels of PGE<sub>2</sub> (b), 13,14-dihydro-15-keto-PGE<sub>2</sub> (c), PGD<sub>2</sub> (d), TXB<sub>2</sub> (e) and (f) PGF<sub>2α</sub>. Plasma 6-keto-PGF<sub>1α</sub> levels in all genotypes compare well with those previously published using enzyme immunoassay measurements. n=6. *, p<0.05 by 1-way ANOVA with B...
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