PodΔ<b><i>Myh9</i></b><b> results in hypersensitivity to HIV nephropathy from transgene </b><b><i>Tg26</i></b><b>.</b>

<p>(A) SDS-PAGE gel with coomassie stain of urine from 24 week old mice from littermates of Tg26+ crosses as outlined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067839#pone-0067839-t002" target="_blank">Table 2</a>. TKO = triple knockout, TH = triple heterozygous control, and Tg = Tg26/+; f/+ “only” genotype. M = molecular weight marker, and BSA are lanes of albumin standards in ug per lane. The TKO urines have more albuminuria, although the results are variable and not normalized to urine concentration with creatinine. (B) Quantification of albuminuria from mouse urine at weeks 20–28 of the indicated genotypes assessed by plate ELISA and normalized to urine creatinine. Yaxis = the ratio of urine albumin to urine creatinine (ug/mg). Beneath the abscissa, N = number of mice assessed for each genotype and each timepoint. Two findings are of statistical significance. First, within each month, the TKO mice have a significantly higher urine ACR than control littermates (*); for 20 weeks, TKO vs. TH (p = 0.0349), and TKO vs. Tg (p = 0.0277); for 24 weeks, TKO vs. TH (p = 0.0127), while the TKO vs. Tg showed a trend (p = 0.0575); for 28 weeks, TKO vs. TH (p = 0.0080) and TKO vs. Tg 0.0109). Secondly, the urine ACR in TKO mice increases over time (#) such that there is a significant difference between the 20 and 28 week TKO (p = 0.019), and a significant difference between the 24 and 28 week TKO (p = 0.0390). Statistical analyses employed unpaired t-test without the assumption of equal variances (Welch correction).