No large-scale genome rearrangements are detected in chromosome III-lost lines.

<p>(A) DNA content of chromosome III-lost lines was similar to that of diploid cells. Cells were stained with Sytox Green and analyzed by fluorescence-activated cell sorting. The first and second panels are haploid and diploid control cells, respectively. The bottom three panels show three examples of chromosome III-lost lines. (B) No obvious chromosomal rearrangements are detected in chromosome III-lost lines. The karyotypes of 14 chromosome III-lost clones (c1 to c14) were analyzed using pulsed-field gel electrophoresis. Southern blot showed hybridization using a probe from the left arm of chromosome III (from 53,372 to 54,379 bp). M, yeast chromosomal DNA marker from a standard laboratory strain; wt, wild type cells used in our experiments.