Analysis of the (pre-) rRNAs contained in various affinity purified LSU (precursor) particles.

<p>Yeast strains Y1878, Y1879, Y2398, Y2404, Y2410 and Y485 expressing the indicated TAP-tag fusion proteins (<b>A</b>) or carrying a C-terminally FLAG-tagged allele of RPS26 under control of the galactose-inducible GAL1/10 promoter (<b>B</b>) were cultivated to exponential growth phase in galactose-containing medium (YPG). The tagged proteins were affinity purified from total cellular extracts using rabbit IgG coupled to magnetic beads (<b>A</b>) or an anti FLAG M2 matrix (<b>B</b>) as described in the materials and methods. (pre-) rRNA species from total cellular extracts (Input) and affinity purified fractions (IP) were analyzed by northern blotting using oligonucleotide probes indicated on the right. Equal signal intensities of the Input and bead (IP) fractions indicate 3% (<b>A</b>) or 6% (<b>B</b>) co-purification efficiences of the respective (pre-) rRNA with bait proteins.