<i>O</i><i>. majorana</i> extract inhibits the migration of the MDA-MB-231 human breast cancer cells.

<p>(A) Confluent culture of MDA-MB-231 cells were wounded by scratching with a pipette tip and the cells were incubated in DMEM supplemented with 10% fetal bovine serum without and with indicated concentrations of OME. The wound was measured with an inverted microscope (x 100 magnification) and photographed. (B) Wound healing assay showing that OME inhibited the migration of MDA-MB-231 breast cancer cells in dose-dependent manner. Values represent means ± SEM (n=3) of the difference between the measurements at time zero and the 4-10 h time period considered. (C) Cell viability (in percent) determined by using CellTiter-Glo Luminescent Cell Viability assay, after incubation of the exponentially growing cells with or without various concentrations of OME for 10 h. (D) Boyden chamber transwell assay indicating that OME inhibited the migration of MDA-MB-231 cells. (E) Quantification of Migrated MDA-MB-231 cells. Migrating cells from at least 5 different random fields were counted under a microscope. Values represent means ± SEM, n=3. Student’s t test was performed to determine the significance (*<i>p</i> < 0.05, **<i>p</i> < 0.005 and ***<i>p</i> < 0.0005).