ER proteins are associated with CLD.

<p>(A) Quantitative immunoblot analysis of GRP78 levels in liver extracts from non-fasted male mice (Control) and fast-fed male mice on LF- or HF-diets. Insets show immunoblots of 50 μg of total liver homogenate protein from 3 mice probed with antibodies to GRP78 or β-actin. The graph shows the average (± SD) GRP78 levels normalized to β-actin from Control (3), LF- (LFD) (N = 3) and HF- (HFD) (N = 3) refed mice. (B) Quantitative immunoblot analysis of GRP78 levels in enriched CLD protein extracts from fast-fed male mice on LF- or HF-diets. Insets show immunoblots of 25 μg of CLD protein from 3 mice. The graph shows the average (± SD) GRP78 levels normalized to 25 μg of CLD protein from LF- (LFD) (N = 3) and HF- (HFD) (N = 3) refed mice. (C) CLD GRP78 levels normalized to CLD TG content. Values are means (± SD) for LFD (N = 3) and HFD (N = 3) refed animals. (D) Representative confocal immunofluorescence images of liver sections from HF refed mice stained with antibodies to Plin2 (red) and GRP78 (green). (E) Representative confocal immunofluorescence images of liver sections from HF refed mice stained with antibodies to Plin2 (red) and PDI (green). Nuclei in images in D and E (blue) were stained with DAPI. Arrows in D and E indicate localization of GRP78 or PDI on CLD. Bar is 10 μm.