Diet effects on metabolism and hepatic lipid storage.

<p>(A) Effects of LF- and HF-refeeding on energy intake in fasted male mice. Values are means (± SD) for 4 animals in each group. Asterisk indicates HFD values differ from LFD values (p<0.01). (B) Energy usage in non-fasted, fasted and LF- and HF-refed male mice as determined by RER. Non-fasted and fasted values correspond to averages (± SD) of 8 animals obtained prior to refeeding. LF- and HF-refeeding values correspond to averages (± SD) for 4 animals in each group. Asterisk indicates values differ from non-fasted controls (p<0.001); dagger indicates values differ from non-fasted and fasted values (p<0.001); double dagger indicates values differ from LF refed and non-fasted values (p<0.001). (C) Representative images of frozen liver sections from LF- and HF-refed male stained with BODIPY and imaged by laser confocal (BODIPY) and CARS microscopy (200X magnification). Asterisks indicate central veins. (D) Representative surface-view of 3D projection images of single cells within liver sections from LF (LFD)- and HF (HFD) -refed mice obtained at 600X magnification. Nuclei are shown in blue.