Comparison of binding to Dyn2 and tetherin for Nef substitutions with impaired infectivity enhancement.

a<p>Infectivity enhancement was calculated as the relative infectivity of Δ<i>nef</i>-viruses <i>trans</i>-complemented with these Nef mutants to Δ<i>nef</i>-viruses <i>trans</i>-complemented with wild-type Nef.</p>b<p>Binding to dynamin 2 was calculated as the relative band intensity of Nef to the band intensity of dynamin 2 in immunoprecipitated samples.</p>c<p>Binding to tetherin was calculated as the relative band intensity of Nef to the band intensity of tetherin in immunoprecipitated samples.