Lung pathology.

<p>Fourty-eight hours after infection Tie2-EPCR mice showed increased lung damage compared to WT mice as reflected by increased mean histological scores as described in the <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002306#s2" target="_blank">Methods</a> section (A). Both WT (B) and Tie2-EPCR (C) mice infected with 300 CFU of <i>B. pseudomallei</i> showed inflammatory infiltrates in the lungs characterized by interstitial inflammation together with necrosis, endothelialitis, bronchitis, edema, thrombi and pleuritis 48 hours after inoculation (H&E staining ×100). Increased granulocyte migration in Tie2-EPCR mice infected with <i>B. pseudomallei</i> was seen 48 hours after infection (D). Representative photographs of Ly-6G-immunostaining (original magnification ×100) for granulocytes of WT (E) and Tie2-EPCR (F) mice showing significantly increased neutrophil influx at 48 h post-inoculation. Tie2-EPCR mice showed increased myeloperoxidase (MPO) levels compared to WT (G) in lung homogenates 48 and 72 h after infection. Data are expressed as box and whisker plots showing the smallest observation, lower quartile, median, upper quartile and largest observation. Numbers of granulocytes are expressed as the percentage of the total lung surface area. Grey boxes represent WT mice, white boxes represent Tie2-EPCR mice (<i>n</i> = 8–9 mice per group for each time point). *<i>P</i><0.05 and **<i>P</i><0.01 for WT versus Tie2-EPCR mice (Mann-Whitney <i>U</i> test).