<i>Cryptococcus</i>-induced perforin loss in YT cells is greater in pH 6.6 compared to pH 7.4.

<p>(A) YT cells were cultured alone or with live <i>Cryptococcus</i> (B3501 in upper panel and R265 in lower panel, E∶T, 1∶10) in pH 7.4 and 6.6 for 20 hours. Cells were harvested, made permeable and immunolabeled with either anti-perforin or isotype control antibodies. Data are representative of three experiments. (B) Reduction in perforin MFI was calculated by deducting the MFI of <i>Cryptococcus</i> stimulated YT from the MFI of unstimulated YT. (C) Same as A except for heat killed B3501. Data are representative of three experiments. (D) YT cells were pretreated for 2 h with 10 nM concanamycin A (B3501+YT+CMA) and anticryptococcal activity was compared with untreated group (B3501+YT). ***, p<0.0001. Data are representative of three experiments.