Embryonic expression and localization of Loh and Prc.

<p>(<b>A–F</b>) Whole mount <i>in situ</i> hybridization revealing the expression patterns of <i>loh</i> (A–C) and <i>prc</i> (D–F) during embryogenesis. Chordotonal organs (double arrowhead in C), the ring gland (arrowhead in E, F) and oenocytes (double arrowhead in F) are indicated. (<b>G, H</b>) Double labeling of <i>loh</i> transcripts and Tinman (G) or <i>odd</i>-LacZ (H) in stage 17 embryos. (<b>I, J</b>) Co-staining of either Prc (I) or Loh (J) with Vkg::GFP in stage 17 embryos demonstrates localization within the cardiac ECM at the basal side of cardiomyocytes. (<b>K, L</b>) Co-staining of Loh and Prc shows co-localization of both proteins.