Rupatadine attenuates cellular senescence in fibrotic mice.

<p>(<b>A</b>) Rupatadine (6.0 mg/kg per day) attenuated the expression or activity of senescence-related molecules in fibrotic lung tissue. Lung tissue extract was prepared for western blotting. The data are representative immune blots and expressed as the mean ± SEM of 8 mice per group. (<b>B</b>) Rupatadine (6 mg/kg) reduced the number of senescent cell in fibrotic mouse lung tissue. Representative images of lung sections were acquired by confocal microscopy for DAPI (blue)/ γ-H2A.X (green) or immunohistochemistry for p21. Scale bar in images = 10 μm or 50 μm. The data are expressed as the mean ± SEM of 5 mice per group. ^#<i>P</i><0.05, ^{# #}<i>P</i><0.01 vs. Sham group; ^*<i>P</i><0.05, ^**<i>P</i><0.01 vs. BLM treated group.