Gene expression changes upon infection.

<p>Presented values are calculated with the 2^-ddCt method. This method yields fold-change values for gene expression between defined sample groups (exposed compared to non-exposed). Error bars are standard errors calculated upon averaging dCt values within sample groups and transformed to fold change errors with error propagation. The solid line marks the value 1 and corresponds to no change between groups. Dashed lines mark the values 2 and 0.5, corresponding to doubled and halved gene expression upon treatment, respectively. Asterisks mark significance of effects as detectable in the univariate outputs of the overall MANOVA (Table S6a in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068181#pone.0068181.s001" target="_blank">File S1</a>). Visualization of fold changes within the two collection sites can be found in Figure S1.