Suspension function comparison of the supercooledrat hepatocytes with other preservation modalities and fresh cells.

<p>A * and ^O means statistically different (p<0.05) than fresh and cryopreserved cells respectively. A) Cell viability after 6 hours of suspension culture; HTS at -4^oC group has significantly higher (p<0.05) viability than all other groups (except fresh cells) on both days. B) Albumin production by cells during 6 hours of cell suspension solution. All groups except cryopreserved cells show similar albumin secretion on day 3 while HTS at -4^oC group produces significantly more (p<0.05) albumin than all other preserved groups on day 5 which is statistically similar (p>0.05) to the fresh cells. C) Urea production by cells during 6 hours of cell suspension. The urea production by all preserved groups except cryopreserved cells is statistically similar (p>>0.05) to fresh cells on day 3; by day 5 of preservation the two groups preserved at -4^oC produce statistically similar levels (p>0.05) of urea to that of fresh cells which is significantly different (p<0.05) than cells preserved at +4^oC. D) Resorufin production by preserved cells using ethoxy coumarin as the substrate (EROD analysis). Enzymatic activity among two non-frozen groups and fresh cells is similar after 3 days of preservation. After 5 days of preservation the HTS at -4^oC group is statistically similar (p>0.05) to fresh cells and significantly better (p<0.05) than UW at +4^oC group. Cryopreserved cells show significantly higher levels of enzymatic activity compared to all other groups.