Effects of ethanol at different concentrations on Rhod-2 fluorescence in cultured neurons.

<p>Murine neocortical neurons were cultured for 8 days, followed by loading of Rhod-2 and subsequent cumulative addition of ethanol at different concentrations 3 min after the addition of 10 µM NMDA. Values are percentages over the maximal fluorescence by A23187 in 3 independent determinations. ^#P<0.05, significantly different from the control value obtained in cells before the addition of ethanol. Statistical significance was determined according to the Students’ <i>t</i>-testanalysis.