Sensitivity of COLO 320 DM total population and CSLCs cells to Oxaliplatin and 5-Fluorouracil following treatment with 25 uM EPA.

<p>(<b>A</b>) COLO 320 DM cells were treated with a range of Oxaliplatin (0.005–0.1 mM) or 5-Fluorouracil (0.05–2 mM) concentrations to determine the inhibitory concentration of 25% (IC25) and 50% (IC50). (<b>B</b>) Cells from COLO 320 DM total population were pre-treated for 48 hours with 25 uM EPA or SA. Afterwards cells were exposed for 24 hours with Oxaliplatin (<i>IC25, 2.5 </i><i>uM; IC50, 10 </i><i>uM</i>) and 5 Fluorouracil (<i>IC25, 100 </i><i>uM; IC50, 1.5 </i><i>mM</i>). (<b>C</b>) CD133 (+) cells were magnetically sorted from the total population of COLO 320 DM and were pre-treated for 48 hours with 25 uM EPA or SA and then exposed for 24 hours to IC25 and IC50 of Oxaliplatin and 5-Fluorouracil. Results represent the mean ± SD of at least three experiments. <i>p</i> values were calculated with Student's t-test on treated samples vs. CTRL VH (* p≤0.05, ** p≤0.01, *** p≤0.001).