Oxidative stress is reduced in the kidneys of diabetic ApoE/GPx1 dKO mice after treatment with m-hydroxy ebselen.

<p><b>A)</b> Kidneys of dKO mice were immunostained with antibody to nitrotyrosine (stained brown) to detect peroxynitrite-induced damage to the tissue. Diabetes resulted in an increase in nitrotyrosine immunostaining, particularly within the tubules. <b>B)</b> Histogram showing that both m-hydroxy ebselen (ME) and ebselen (Eb) treatment attenuated nitrotyrosine immunolabelling in the tubules of diabetic dKO kidneys. <b>C)</b> Histogram showing the urinary levels of 8-hydroxy-2-deoxy guanosine (8-OHdG) measured by an EIA kit. Values are normalised to urinary creatinine levels and expressed as µg/mmol. *<i>P</i><0.05, **<i>P</i><0.01 vs ND control; ^#<i>P</i><0.05, ^##<i>P</i><0.01 vs diabetic kidney. ND = non diabetic; ME = m-hydroxy ebselen; Diab = diabetic; Eb = ebselen.